Previous research has shown that the active ingredients of PPM™ penetrate the fungus or the bacterium cell wall and inhibit the activity of key enzymes in the citric acid cycle and in the Electron Transport Chain. PPM™ is a broad-spectrum preservative and biocide, which kills bacteria and fungi cells, prevents germination of spores, and in higher concentrations, can eliminate explants of endogenous contamination. Guri worked with the Volcani Agricultural Research Center in Israel, Michigan State University in East Lansing, Michigan and DNAP in New Jersey. Before joining Plant Cell Technology, Inc., Dr. Assaf Guri holds degrees in genetics, applied genetics and plant breeding from the Hebrew University in Jerusalem and Michigan State University in the US. The principal PCT scientist involved in the development of the PPM™ application is Dr. While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also - in many cases - be used to reduce endogenous contamination. PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics. It is heat stable and therefore can be autoclaved with the media. PPM™ prevents the germination of both bacteria and fungi spores. At optimum doses, PPM™, which stands for Plant Preservative Mixture, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.ĭespite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches. Plant Cell Technology has developed a new, heat stable preservative/biocide, which effectively prevents or reduces microbial contamination in plant tissue culture.
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